BioImaging Center Lille Facility (BICeL)
Plateformes Lilloises en Biologie & Santé – PLBS – UMS 2014 – US 41
Presentation
The BioImaging Center Lille (BICeL) is the academic platform for imaging, histology and cytometry of the University Hospital Campus, the Faculty of Science and Technology and the Pasteur Institute of Lille.
The BICeL brings together expertise, know-how and equipment on four platforms: (1) cytometry & flow imaging, (2) histology, (3) biophotonics and (4) electronic microscopy. Research & development in imaging techniques extends from the cell, to tissues and small rodent and aquatic organisms. The Pasteur Campus site offers access to very high technology instruments such as a scanning electron microscope for (cryo)tomography, a flow imager, microscopes in confinement security labs for fast, high-resolution video and intravital imaging. BICeL belongs to the service unit PLBS – UMS 2014 – US 41 (https://ums-plbs.univ-lille.fr/).
Highlights
- The Facility has benefited from fundings from the EquipEx Investment for the Future Program (ImagInEx BioMed) supported by European Regional Developmental Funds and also from the CPER Longevity and support from the University of Lille. The scientific developments are carried out in particular in super-resolution biophotonic microscopy and correlative microscopy with the F Lafont team at the Infection and Immunity Center of Lille. The facilities are open to requests from academic and private laboratories.
Members
Frank LAFONT
DR CNRS / IPL, scientific Director of BioImaging Center Lille (BICeL), scientific manager
Marc MONGY
Engineer IPL, technical manager Image Analysis
Sophie SALOMÉ
Technical manager Photonic Microscopy incl. Intravital Microscopy
Elisabeth WERKMEISTER
Research engineer CRNS, Image analysis and super-resolution microscopy manager
Nicolas BAROIS
Research Engineer Inserm, technical manager
Hélène BAUDERLIQUE
Research engineer IPL, technical manager
Christelle FAVEEUW
CR Inserm, scientific manager
Publications
Ciczora Y, Janel S, Soyer M, Popoff M, Werkmeister E, Lafont F.
Blocking bacterial entry at the adhesion step reveals dynamic recruitment of membrane and cytosolic probes.
Biol Cell. 2019 Mar;111(3):67-77.
Janel S, Popoff M, Barois N, Werkmeister E, Divoux S, Perez F, Lafont F.
Stiffness tomography of eukaryotic intracellular compartments by atomic force microscopy.
Nanoscale 11:10320-10328.
Venugopal K, Chahade S, Werkmeister E, Baoirs N, Periz J, Lafont F, Tardieux I, Khalife J, Langsley G, Meissner M, Marion S.
Rab11A regulated dense granule transport and secretion during Toxoplasma gondii invaions of host cell and parasite replication.
2019 PLoS Pathogen 16:e1008106
Sencio V, Barthelemy A, Tavares LP, Machado MG, Soulard D, Cuinat C, Queiroz-Junior CM, Noordine ML, Salomé-Desnoulez S, Deryuter L, Foligné B, Wahl C, Frisch B, Vieira AT, Paget C, Milligan G, Ulven T, Wolowczuk I, Faveeuw C, Le Goffic R, Thomas M, Ferreira S, Teixeira MM, Trottein F.
Gut Dysbiosis during Influenza Contributes to Pulmonary Pneumococcal Superinfection through Altered Short-Chain Fatty Acid Production.
Cell Rep. 2020 Mar 3;30(9):2934-2947.e6. doi: 10.1016/j.celrep.2020.02.013.
Trzaska C, Amand S, Bailly C, Leroy C, Marchand V, Duvernois-Berthet E, Saliou JM, Benhabiles H, Werkmeister E, Chassat T, Guilbert R, Hannebique D, Mouray A, Copin MC, Moreau PA, Adriaenssens E, Kulozik A, Westhof E, Tulasne D, Motorin Y, Rebuffat S, Lejeune F.
2,6-Diaminopurine as a highly potent corrector of UGA nonsense mutations.
Nat Commun. 2020 Mar 20;11(1):1509. doi: 10.1038/s41467-020-15140-z.
Keywords
Transmission & Scanning Electronic Microscopy ; Intravital microscopy ; Correlative microscopy ; Flow cytometry & imaging