Peptide Chemistry

La CSB platform offers cutting-edge expertise in two complementary fields.

The first area of ​​expertise concerns the semi-synthesis or total synthesis of peptides and proteins. The use of native peptide ligation chemistries, such as native chemical ligation or bis(2-sulfanylethyl)amido peptide ligation (SEA), allows access to complex peptide scaffolds (cyclic, branched, polycyclic, etc.) or proteins with precise control over their composition and structure. The starting peptide segments are produced by conventional solid-phase peptide synthesis or by recombinant methods. They are assembled in solution or on the solid phase depending on the final application.

Our expertise includes the folding of synthesized proteins and the determination of disulfide bonds, when present, using proteomic methods (enzymatic digestion followed by mass spectrometry analysis). The secondary structure of proteins can be studied using circular dichroism.

The second area of ​​expertise offered by the CSB platform is the study of peptide and protein activity using a range of biochemical, biophysical, and cell-based assays. Our expertise includes the development of customized assays to study protein-protein and other biomolecular interactions using AlphaScreen® technology. We are also equipped to identify protein-binding partners from biological extracts. The production of biotinylated proteins by chemical synthesis is particularly relevant in this context and is a major asset for this type of study. As another example, we develop phenotypic cell-based assays (cell dispersion, cell morphogenesis, etc.) to study the activity of peptides, proteins, or small molecules.