Lille Platforms in Biology & Health – PLBS – UMS 2014 – US 41
The Proteomics and Modified Protein Analysis Platform (P3M) is a technological platform of the Pasteur Institute of Lille, part of the Joint Service Unit PLBS -UMS 2014 – US 41.
P3M offers expertise ranging from proteome identification by nanoLC-MS/MS and the use of databases or de novo analysis, to their quantification with or without labeling. The available equipment also allows the study of small post-translational modifications (PTMs) with or without enrichment, as well as the characterization of protein maturation events.
The analysis of the PTM of a protein can be carried out at the peptide level, but also by analysis of the mass of the whole protein by LC-MS.
P3M also has native mass expertise for analyzing interactions between proteins, ligands or nucleic acids.
News
- January 2020: Integration of P3M into the UMS PLBS.
- June 2020: Development of the Thermal Profiling method for proteins by TMT quantification.
- September 2020: Arrival of AS Lacoste.
- January 2021: Collaboration labeling between P3M and PAGés (Glycoconjugate Analysis Platform) by the Infrastructure in Biology, Health and Agronomy (GIS IBiSA).
Cross-functional projects
Members
Jean-Michel Saliou
Research engineer, team leader
Anne-Sophie LACOSTE
UL Research Engineer
Keywords
Team contact
Jean-Michel Saliou
Research engineer, team leader
Publications
Common Biol. 2021 Jan 8;4(1):46. doi:10.1038/s42003-020-01580-2. PMID: 33420409.
Streamlined copper defenses make Bordetella pertussis connecting on custom-made operon.
Nat Commun. 2020 Mar 20;11(1):1509. doi:10.1038/s41467-020-15140-z. PMID: 32198346; PMCID: PMC7083880.
2,6-Diaminopurine as a highly potent corrector of UGA nonsense mutations.
SciRep. 2019 Dec 5;9(1):18378. doi:10.1038/s41598-019-54935-z. PMID: 31804568; PMCID: PMC6895148.
Identification of potential chemosignals in the European water flies Arvicola terrestris.
Nucleic Acids Res. 2018 Jul 6;46(12):6057-6068. doi:10.1093/nar/gky373. PMID: 29788176; PMCID: PMC6159514.
Cooperative binding of ApiAP2 transcription factors is crucial for the expression of virulence genes in Toxoplasma gondii.
Gastroenterology. 2018 Jan;154(1):211-223.e8. doi: 10.1053/j.gastro.2017.09.020. Epub 2017 Sep 25. PMID: 28958858.
